This Gândhârî version of the Buddha's Discourse on Not-self (P Anattalakkhaṇa-sutta, Skt. *Anâtmalakṣaṇa-sûtra) is the second of six sûtras or discourses preserved on scroll 22 of the Robert Senior collection. The Gândhârî title *Aṇatvalakṣaṇa Sutra has been reconstructed on the basis of the title given in the Pali parallels. This discourse records the second teaching given by the Buddha shortly after his awakening to the group of five monks at the Deer Park near Benares. The significance of this teaching and its exposition of the five aggregates (G kadha, P khandha, Skt. skandha) as having the nature of "not-self" (G aṇatva, P anatta, Skt. anâtman) is indicated by the numerous versions available in Pali, Sanskrit, Tibetan, and Chinese, and now Gândhârî. This publication contains the first diplomatic edition, reconstruction, and translation of this Gândhârî sûtra version.