Drosophila simulans: A species with improved resolution in evolve and resequence studies

Neda Barghi, Raymond Tobler, Viola Nolte, Christian Schlotterer

    Research output: Contribution to journalArticle

    Abstract

    The combination of experimental evolution with high-throughput sequencing of pooled individuals-i.e., evolve and resequence (E&R)-is a powerful approach to study adaptation from standing genetic variation under controlled, replicated conditions. Nevertheless, E&R studies in Drosophila melanogaster have frequently resulted in inordinate numbers of candidate SNPs, particularly for complex traits. Here, we contrast the genomic signature of adaptation following ~60 generations in a novel hot environment for D. melanogaster and D. simulans. For D. simulans, the regions carrying putatively selected loci were far more distinct, and thus harbored fewer false positives, than those in D. melanogaster. We propose that species without segregating inversions and higher recombination rates, such as D. simulans, are better suited for E&R studies that aim to characterize the genetic variants underlying the adaptive response.
    Original languageEnglish
    Pages (from-to)2337-2343
    JournalG3: Genes, Genomes, Genetics
    Volume7
    Issue number7
    DOIs
    Publication statusPublished - 2017

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